Ref ID: 8612
Ref Type: Journal
Authors: Shepherd, M., Cross, M. J., Stokoe, R. L., Scott, L. J., and Jones, M. E.
Pub Date: 2002
Journal Name: Plant Molecular Biology Reporter
Volume: 20
Issue: 4
Start Page: 1
End Page: 10
ISBN/ISSN: 1335-048X
Keywords: AFLP/Araucaria/Australia/biology/Pinus caribaea/Eucalyptus cloeziana/Araucaria cunninghamii/DNA/Pinus elliottii/Eucalyptus/extraction/forest/Genera/genetics/Pinus caribaea var.hondurensis/hybrid/method/microsatellite/molecular/pine/Pinus/plant/quality/species/system/tissue/tree/high/molecular genetics/genetic/methodology/commercial/ITS/hoop pine/molecular biology
Abstract: Trees are typically more demanding than other plants to extract pure, high quality DNA for molecular genetics and may not necessarily be amenable to advances in extraction methodology suitable for other plants. A recently available, commercial, high throughput DNA extraction system utilising a silica binding matrix for purification and a multi-sample mixer mill for tissue disruption was evaluated for its suitability with Eucalyptus spp., Pinus spp. and Araucaria cunninghamii (hoop pine). DNA suitable for a range of molecular biology applications was successfully extracted from all genera. The method was highly reliable when tested in over 500 preparations and could be adapted to different tree species with relatively minor modifications to the standard protocols.
Notes: Entered by mshepher (23/4/2001):
Reprint: Not in File
Program: SPF Genetic Improvement
Project: A7
Deliverable: A7-1; A7-3
Confidentiality: Public
Availability: Authors
Report: Annual Report 2002/3
Type: Article
Address: mshepher@scu.edu.au