Ref ID: 10686
Ref Type: Confidential Report
Primary Title: The collective pine genome is characterised by synteny, paralogy and recombination shrinkage
Primary Authors: Shepherd, M., Williams, C. G., Cross, M. J., Eggler, P., and Henry, R. J.
Secondary Authors:
Secondary Title:
Periodical:
Volume: 139
Issue: 1
Start Page:
End Page: 32
Primary Date: 22-3-2004
Pub Place: Lismore, NSW
Publisher: CRC-SPF
Series Title: Technical Report
Series Authour:
Secondary Date:
Keywords: analysis/barrier/CAD/candidate/candidate genes/characteristic/comparative/comparative genome analysis/comparative mapping/comparison/conservation/evolution/F1/F2/family/gene/generation/genes/genetic/genetic map/genome/genome organisation/high/homologous/hybrid/hybridisation/hybrids/impact/incompatibility/information/interaction/isolation/location/loci/map/marker/mechanism/microsatellite/microsatellites/negative/new/PAL/parology/pine/Pinus caribaea var.hondurensis/Pinus elliotii var.elliotii/Pinus hybrid/population/property/putative/report/reproductive barriers/RFLP/sequence/south-east Queensland/speciation/species/system/Technical Report/transfer/wood/wood properties
Abstract: Comparative genome analysis provides new understanding of genome organisation and evolution. Comparative mapping in hybrids can provide further insight into the mechanisms of reproductive isolation and speciation. Previously we reported a map comparison amongst three species of Australes pines (Shepherd et al., 2003). We found unusually high synteny amongst the three taxa which facilitated the merger of homologous linkage groups into a collective map for the group. The high order of genome conservation detected was not an artefact of the marker system (microsatellites) as it was consistent with previous reports in RFLP and ESTp synteny in hard pines (Brown et al., 2001; Devey et al., 1999; Komulainen, 2003). Despite hypervariability at microsatellites, their flanking sequences are conserved and transfer amongst related taxa. Here we report evidence of higher order conservation in the organisation in microsatellites and the mechanisms of incompatibilities amongst pine species. Microsatellite families were found to be conserved; paralogues predate species' divergence and may be phylogenetically informative. High synteny and collinearity suggested genetic maps will be an efficient conduit for exchange of genetic information in the group. Like species comparisons, hybrid and parental maps also have high synteny but there was evidence subtle species incompatibilities impact on map characteristics. Although there are no gross chromosomal differences amongst parental taxa, small cryptic structural differences apparently contribute to shrinkage in hybrid maps. Map distortion was also indicative of putative inviability or adaptation loci. Different selective forces apparently operated in an F1 than the F2 as segregation distortion was distinct for each generation. Evidence of negative heterospecific gene interactions suggested post-zygotic barriers may be more important in the reproductive isolation and shaping of hybrid populations than in pure species of pines. Genetic map location for two candidate genes for wood properties, CAD and PAL are also reported.
Notes: Entered by Mervyn Shepherd (23/03/2004)
Reprint: In File
Availability: Online
Address: mshepher@scu.edu.au
Program: SPF Genetic Improvement
Project: A7
Deliverable: A7-1; A7-2; A7-3
Confidentiality: Confidential to All Partners
Report: Annual Report 2003/4
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