Ref ID: 10678
Ref Type: Journal
Authors: Eyles, A., Davies, N. W., Mitsunaga, T., Mihara, R., and Mohammed, C. L.
Pub Date: 2004
Journal Name: Forest Pathology
Volume: 34
Issue:
Start Page: 225
End Page: 232
ISBN/ISSN:
Keywords: analysis/antioxidant/antioxidant activity/antioxidant-like activity/assay/defence/electrospray/Eucalyptus/Eucalyptus globulus/extractives/globulus/hydrolysable tannins/In Vitro/ion/mass spectrometry/metabolite/negative/new/sample/secondary metabolite/system/tannin/Tasmania/tree/wood/wound/wound wood
Abstract: Superoxide dismutase-like activity of crude Eucalyptus globulus extracts obtained from new wound wood was measured using the WST-1 assay. This in vitro assay determined the activity of superoxide radicals scavenging in a xanthine oxidase system. The 50% inhibition (IC50) of formazan formation was estimated for fractionated samples and purified compounds isolated from the crude wound wood extract i.e. engelitin and pedunculagin. Standards of gallic acid and pentagalloylglucose were also measured. Fraction II as well as the compounds pentagalloylglucose and pedunculagin showed the highest levels of antioxidant activity. Subsequent analysis of Fraction II by HPLC coupled to negative ion electrospray mass spectrometry revealed the fraction to be dominated by hydrolysable tannins including pedunculagin, di-, tri- and tetragalloylglucoses. These preliminary results provide some evidence for an antioxidant role of secondary metabolites in tree wound repair and defence
Notes: Entered by Alieta Eyles (17/03/2004)
Reprint: Not in File
Program: SPF Resource Protection
Project: C5
Deliverable: C5-3; C5-3
Confidentiality: Public
Availability: Authors
Report: Annual Report 2003/4
Type: Article
Address: Alieta.Eyles@dpiwe.tas.gov.au