Ref ID: 10676
Ref Type: Confidential Report
Primary Title: Genetic architecture of adventitious rooting in tropical hybrid pines
Primary Authors: Shepherd, M., Eggler, P., Cross, M. J., Mellick, R., Dieters, M. J., Toon, P. G., Dale, G. T., and Henry, R. J.
Secondary Authors:
Secondary Title:
Periodical:
Volume: 132
Issue:
Start Page:
End Page: 42
Primary Date: 3-3-2004
Pub Place: Lismore, NSW
Publisher: CRC-SPF
Series Title: Technical Report
Series Authour:
Secondary Date:
Keywords: adventitious rooting/allele/architecture/average effect/clonal forestry/clonal propagation/clone/cutting/cuttings/data/detection/distribution/effect/efficiency/expression/F1/F2/family/gene/genes/genetic/genetic map/genetic markers/genotype/Gympie,Queensland/hybrid/hybrids/improvement/inbreeding/initiation/loci/map/marker/marker-aided selection/microsatellite/microsatellites/N/natural/New South Wales/P/parameter/pine/Pinus/Pinus caribaea/Pinus caribaea var.hondurensis/Pinus elliotii/Pinus elliotii var.elliotii/Pinus elliottii/Pinus hybrid/population/QTL/quantitative trait/Queensland/rate/region/report/root/sample/selection/selective genotyping/species/stability/Technical Report/vegetative cuttings
Abstract: Genetic architecture of strike rate (adventitious root initiation in vegetative cuttings) was examined in two unrelated hybrid pine families (Pinus elliottii x P. caribaea), an outbred F1 and an inbred F2. The approach was to selectively genotype distribution tails (n=32) from large families of clones (n = 287 and 356 clones for F1 and F2 respectively) for microsatellite markers distributed across a genetic map to identify genomic regions associated with strike rate. QTL parameters were then estimated more accurately on a larger random sample (n = 244 and 192 clones) from each family. Validation of QTL for strike rate was achieved by virtue of synteny in two independent populations. An oligogenic QTL architecture for strike rate was apparent for both families. A predominance of favourable genes and alleles from P. caribaea was also consistent with this species as noted as the better species for adventitious rooting. A predominance of additive gene action at all loci in the inbred F2 may explain stability of gene expression at different levels of inbreeding. The detection of moderate to large effects that were stably expressed in different genetic backgrounds, was suggestive of species effects i.e. genes which have become fixed for divergent alleles in natural populations of the parental species. Our data suggested some species effects have large average effects and could be used for population improvement. This suggested there should be efficiencies for marker-aided selection in hybrids that may not be available in pure species populations
Notes: Entered by Mervyn Shepherd (02/03/2004)
Reprint: In File
Availability: Online
Address: mshepher@scu.edu.au
Program: SPF Genetic Improvement
Project:
Deliverable: A7-3; A7-3
Confidentiality: Confidential to All Partners
Report: Annual Report 2003/4
Misc 1:
Misc 2:
Misc 3:
ISBN/ISSN: