Ref ID: 10669
Ref Type: Journal
Authors: Patterson, B., Gore, P. L., Potts, B. M., and Vaillancourt, R. E.
Pub Date: 2004
Journal Name: Australian Journal of Botany
Volume: 52
Issue: 6
Start Page: 781
End Page: 788
ISBN/ISSN:
Keywords: allozyme/analysis/cost/cutting/cycle/deployment/dominance/effect/Eucalyptus/Eucalyptus globulus/Eucalyptus globulus ssp.globulus/family/flower/full-sib/genetic/globulus/isolation/Labill/low-cost/marker/microsatellite/new/open-pollination/paternity/plantation/plantations/pollen/pollination/production/progeny/seed/seed production/seedling/seedlings/selfing/show/STEPS/style/technique/tree/trees/volume/CSIRO
Abstract: The effectiveness of hand supplementary pollination (HSP) for large-scale production of elite Eucalyptus globulus Labill. seed was tested using pollen fixed for a rare allozyme. We assayed the paternity of 1,954 seedlings derived from 1089 flowers on 14 trees with no emasculation or style isolation. We first compared the success of pollinating the transversely cut-style surface to direct stigma pollination. For flowers pollinated up to six days after operculum shed, style cutting resulted in a 15-fold increase in the number of seed with the marker allozyme (intended pollination) compared to stigma pollination. There was no difference between either pollination treatment or open pollination in 7-14 day-old flowers. We subsequently pollinated 13 trees using the cut-style technique that resulted in 86.9% of progeny having the marker allozyme. Microsatellite analysis showed that only 4.8% of the progeny were selfs. Thus we show that it is possible to obtain seed with low level of contamination and little selfing, without the costly steps of flower emasculation, isolation and labelling, by simply cutting the style, just prior to pollination, on a weekly cycle. Elite full-sib families can be generated at low cost for deployment into plantations to exploit both additive and dominance genetic effects
The full-text link provided is a pre-publication version of an article published in the Australian Journal of Botany in the volume stated in this citation. The publisher of the Australian Journal of Botany is CSIRO Publishing, http://www.publish.csiro.au/?nid=65
Notes: Entered by Rene Vaillancourt (29/01/2004)
Reprint: In File
Program: SPF Genetic Improvement
Project: A1
Deliverable: A1-3.2
Confidentiality: Confidential to All Partners
Availability: Authors
Report: Annual Report 2004/5
Type: Article
Address: Rene.Vaillancourt@utas.edu.au